2APJ

X-Ray Structure of Protein from Arabidopsis Thaliana AT4G34215 at 1.6 Angstrom Resolution

Replaces:  2AEA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2005-08-10
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.98245
Spacegroup name	P 1
Unit cell lengths	40.688, 71.918, 93.213
Unit cell angles	108.16, 93.25, 90.40

Refinement procedure

Resolution	35.852 - 1.600
R-factor	0.14746
Rwork	0.146
R-free	0.18300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PHYRE SERVER HOMOLOGY MODEL BASED ON 1ZMB
RMSD bond length	0.018
RMSD bond angle	1.617
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (refmac_5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.640
High resolution limit [Å]	1.600	1.600
Rmerge	0.047	0.240
Number of reflections	125043
<I/σ(I)>	17.57	4.611
Completeness [%]	94.5	83.7
Redundancy	3.8	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	273	PROTEIN SOLUTION (10 MG/ML PROTEIN, 0.005 M BIS-TRIS, 0.050 M SODIUM CHLORIDE, 0.003 M SODIUM AZIDE, 0.0003 M TCEP, PH 6.0) MIXED IN A 1:1 RATIO WITH THE WELL SOLUTION (18% PEG 4000, 0.010 M POTASSIUM NITRATE, 0.050 M MES, 0.050 M SODIUM ACETATE PH 5.5), vapor diffusion, hanging drop, temperature 273K
1	VAPOR DIFFUSION, HANGING DROP	5.5	273	PROTEIN SOLUTION (10 MG/ML PROTEIN, 0.005 M BIS-TRIS, 0.050 M SODIUM CHLORIDE, 0.003 M SODIUM AZIDE, 0.0003 M TCEP, PH 6.0) MIXED IN A 1:1 RATIO WITH THE WELL SOLUTION (18% PEG 4000, 0.010 M POTASSIUM NITRATE, 0.050 M MES, 0.050 M SODIUM ACETATE PH 5.5), vapor diffusion, hanging drop, temperature 273K
1	VAPOR DIFFUSION, HANGING DROP	5.5	273	PROTEIN SOLUTION (10 MG/ML PROTEIN, 0.005 M BIS-TRIS, 0.050 M SODIUM CHLORIDE, 0.003 M SODIUM AZIDE, 0.0003 M TCEP, PH 6.0) MIXED IN A 1:1 RATIO WITH THE WELL SOLUTION (18% PEG 4000, 0.010 M POTASSIUM NITRATE, 0.050 M MES, 0.050 M SODIUM ACETATE PH 5.5), vapor diffusion, hanging drop, temperature 273K