2AP2

SINGLE CHAIN FV OF C219 IN COMPLEX WITH SYNTHETIC EPITOPE PEPTIDE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1999-03
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	68.792, 82.659, 93.978
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.400
R-factor	0.231 *
Rwork	0.221
R-free	0.27400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ap2
RMSD bond length	0.005
RMSD bond angle	26.760 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (0.5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.460
High resolution limit [Å]	2.400	2.400
Rmerge	0.084 *	0.472 *
Total number of observations	142245 *
Number of reflections	21637	1267 *
<I/σ(I)>	23.1	2.6
Completeness [%]	98.7	88.8
Redundancy	15.1	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.3		THE PROTEIN WAS CRYSTALLIZED IN 40% MPD, 50 MM MES PH 6.3

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	MPD	40 (%(v/v))
2	1	drop	MES	50 (mM)