2AP1
Crystal structure of the putative regulatory protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-07-21 |
Detector | SBC-3 |
Wavelength(s) | 1.284 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 81.581, 105.744, 88.262 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.900 |
R-factor | 0.1694 |
Rwork | 0.168 |
R-free | 0.20508 |
Structure solution method | SAD |
RMSD bond length | 0.016 |
RMSD bond angle | 1.643 |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.049 | 0.232 |
Number of reflections | 30297 | |
<I/σ(I)> | 15.1 | 7.9 |
Completeness [%] | 99.6 | 99.9 |
Redundancy | 6.8 | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 295 | HEPES, tri-Sodium Citrate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K |