2ANX
crystal structure of bacteriophage P22 lysozyme mutant L87M
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-10-10 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 134.013, 50.415, 46.587 |
| Unit cell angles | 90.00, 103.80, 90.00 |
Refinement procedure
| Resolution | 42.000 - 1.040 |
| R-factor | 0.1169 |
| Rwork | 0.117 |
| R-free | 0.14990 |
| Structure solution method | SAD |
| RMSD bond length | 0.020 |
| RMSD bond angle | 0.035 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXCD |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.100 | 1.080 |
| High resolution limit [Å] | 1.040 | 1.040 |
| Rmerge | 0.053 | 0.222 |
| Number of reflections | 144532 | |
| <I/σ(I)> | 42.3 | 3.9 |
| Completeness [%] | 99.6 | 99.4 |
| Redundancy | 4.4 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 277 | PEG 3350, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K, pH 7.50 |
