2ANI
Crystal structure of the F127Y mutant of Ribonucleotide Reductase R2 from Chlamydia trachomatis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-03-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.098 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 62.560, 62.560, 171.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| R-factor | 0.167 |
| Rwork | 0.164 |
| R-free | 0.22600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.540 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 23732 | |
| <I/σ(I)> | 14.1 | 4.1 |
| Completeness [%] | 99.3 | 100 |
| Redundancy | 4.95 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.3 | 298 | PEG 20K, MES, PbCl2, pH 6.3, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
