2ANG

CRYSTAL STRUCTURE OF HUMAN ANGIOGENIN OF THE MET(-1) FORM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.5
Synchrotron site	SRS
Beamline	PX9.5
Temperature [K]	288
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	C 2 2 21
Unit cell lengths	82.900, 118.980, 37.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.000
R-factor	0.217 *
Rwork	0.217
R-free	0.28600
Structure solution method	OTHER
Starting model (for MR)	1ang
RMSD bond length	0.010
RMSD bond angle	1.800
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.100
High resolution limit [Å]	2.000	2.000
Rmerge	0.088	0.374
Total number of observations	141072 *
Number of reflections	13170
<I/σ(I)>	13.5	5.2
Completeness [%]	99.5	99.4
Redundancy	10.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	5.2	16 *	pH 5.2

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	SODIUM CITRATE
2	1	1	SODIUM POTASSIUM TARTRATE
3	1	1	PEG 6000
4	1	2	SODIUM CITRATE
5	1	2	SODIUM POTASSIUM TARTRATE
6	1	2	PEG 6000

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	sodium citrate	10 (mM)
3	1	drop	sodium potassium tartrate	0.1 (M)
4	1	drop	PEG6000	5 (%)
5	1	reservoir	sodium citrate	20 (mM)
6	1	reservoir	sodium potassium tartrate	0.2 (M)
7	1	reservoir	PEG6000	10 (%)