2AN9
Crystal Structure Of Oligomeric E.coli Guanylate Kinase In Complex With GDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-08-04 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 63 |
| Unit cell lengths | 68.225, 68.225, 151.272 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.690 - 2.350 |
| R-factor | 0.18859 |
| Rwork | 0.185 |
| R-free | 0.25762 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ex7 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.478 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.410 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.071 | 0.144 |
| Number of reflections | 16498 | |
| <I/σ(I)> | 28.8 | 16.4 |
| Completeness [%] | 99.3 | 97.7 |
| Redundancy | 12 | 11.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 292 | 4% (w/v) PEG 3350, 40mM potassium thiocyanate, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
