2AN2
P332G, A333S Double mutant of the Bacillus subtilis Nitric Oxide Synthase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F2 |
Synchrotron site | CHESS |
Beamline | F2 |
Temperature [K] | 193 |
Detector technology | CCD |
Collection date | 2004-11-16 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.96 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 80.603, 93.515, 63.571 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.600 |
R-factor | 0.21712 |
Rwork | 0.216 |
R-free | 0.23736 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.013 |
RMSD bond angle | 1.623 |
Phasing software | AMoRE |
Refinement software | REFMAC (5.1.9999) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.640 |
High resolution limit [Å] | 2.600 | 2.600 |
Number of reflections | 45160 | |
<I/σ(I)> | 12.56 | |
Completeness [%] | 98.7 | 69.1 |
Redundancy | 3.62 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | PEG4000, Na Cacodylate, K Acetate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |