2AMX

Crystal structure of Plasmodium Yoelii Adenosine deaminase (PY02076)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2005-07-14
Detector	RIGAKU RAXIS IV
Spacegroup name	P 21 21 21
Unit cell lengths	66.719, 90.332, 137.086
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.900 - 2.020
R-factor	0.197
Rwork	0.196
R-free	0.24600
Structure solution method	SAD
RMSD bond length	0.020
RMSD bond angle	1.686
Data reduction software	CrystalClear
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.900	2.050
High resolution limit [Å]	2.020	2.020
Rmerge	0.103	0.699
Number of reflections	55046
<I/σ(I)>	7.2	2.13
Completeness [%]	99.7	99.1
Redundancy	5.6	5.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5.5	300	20% PEG 3350, 0.1 M Na cacodylate pH 5.5, 0.2 M MgCl2, 10 mM CoCl2, VAPOR DIFFUSION, temperature 300 K, pH 5.50
1		5.5	300	20% PEG 3350, 0.1 M Na cacodylate pH 5.5, 0.2 M MgCl2, 10 mM CoCl2, VAPOR DIFFUSION, temperature 300 K, pH 5.50