2AMV
THE STRUCTURE OF GLYCOGEN PHOSPHORYLASE B WITH AN ALKYL-DIHYDROPYRIDINE-DICARBOXYLIC ACID
Replaces: 1AMVExperimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
Synchrotron site | EMBL/DESY, Hamburg |
Beamline | X11 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1995-11-15 |
Detector | MARRESEARCH |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 127.110, 127.110, 115.460 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.300 |
R-factor | 0.201 |
Rwork | 0.201 |
R-free | 0.28200 |
Structure solution method | OTHER |
RMSD bond length | 0.008 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR |
Refinement software | X-PLOR (3.8) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.095 | 0.382 |
Total number of observations | 242759 * | |
Number of reflections | 39549 | |
<I/σ(I)> | 10.5 | 3.6 |
Completeness [%] | 94.9 | 95.9 |
Redundancy | 6.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | unknown * | 6.7 | 16 * | PHOSPHORYLASE B WAS COCRYSTALLISED WITH 1 MM W1807 IN A MEDIUM CONSISTING OF 27-28 MG/ML ENZYME, 1 MM SPERMINE, 3 MM DTT, 10 MM BES, 0.1 MM EDTA, AND 0.02% SODIUM AZIDE, PH 6.7 (16 DEG C)., temperature 289K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | W1807 | 1 (mM) | |
2 | 1 | 1 | enzyme | 27-28 (mg/ml) | |
3 | 1 | 1 | spermine | 1 (mM) | |
4 | 1 | 1 | dithiothreitol | 3 (mM) | |
5 | 1 | 1 | BES | 10 (mM) | |
6 | 1 | 1 | EDTA | 0.1 (mM) | |
7 | 1 | 1 | sodium azide | 0.02 (%) |