2AMO
Loose Dimer of a Bacillus subtilis Nitric Oxide Synthase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F2 |
Synchrotron site | CHESS |
Beamline | F2 |
Temperature [K] | 193 |
Detector technology | CCD |
Collection date | 2001-10-22 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.92 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 81.314, 93.211, 118.546 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.620 - 2.600 |
R-factor | 0.2756 |
Rwork | 0.274 |
R-free | 0.28969 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1m7z |
RMSD bond length | 0.010 |
RMSD bond angle | 1.812 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.1.9999) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.650 |
High resolution limit [Å] | 2.600 | 2.600 |
Number of reflections | 23234 | |
Completeness [%] | 98.4 | 93.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | Na Cacoldylate, Potassium Acetate, Peg4K, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | Na Cacoldylate, Potassium Acetate, Peg4K, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | Na Cacoldylate, Potassium Acetate, Peg4K, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |