2AML

Crystal structure of Lmo0035 protein (46906266) from LISTERIA MONOCYTOGENES 4b F2365 at 1.50 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2005-07-03
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.97932, 0.90497
Spacegroup name	P 21 21 21
Unit cell lengths	56.315, 101.397, 120.151
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.450 - 1.500
Rwork	0.140
R-free	0.16800
Structure solution method	MAD
RMSD bond length	0.017
RMSD bond angle	1.628
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SHELX
Refinement software	REFMAC (5.2.0013)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.460	1.580
High resolution limit [Å]	1.500	1.500
Rmerge	0.070	0.406
Number of reflections	110766
<I/σ(I)>	7.5	1.8
Completeness [%]	100.0
Redundancy	3.7	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5.6	277	15.0% Glycerol, 0.17M NH4OAc, 25.5% PEG-4000, 0.1M Citrate pH 5.6 , VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K