2AMH

Crystal Structure of Maf-like Protein Tbru21784AAA from T.brucei

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2005-04-03
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9791
Spacegroup name	I 4
Unit cell lengths	97.068, 97.068, 48.894
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	18.000 - 2.000
R-factor	0.199
Rwork	0.197
R-free	0.23100
Structure solution method	MAD
RMSD bond length	0.009
RMSD bond angle	1.071
Data scaling software	SCALA
Phasing software	SOLVE (2.08)
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	18.000	2.110
High resolution limit [Å]	2.000	2.000
Rmerge	0.073	0.224
Number of reflections	15517
<I/σ(I)>	6.5	2.6
Completeness [%]	99.8	99.9
Redundancy	7	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	298	PEG 20K, MnSO4, NaoAc, pH 5, VAPOR DIFFUSION, SITTING DROP, temperature 298K
1	VAPOR DIFFUSION, SITTING DROP	5	298	PEG 20K, MnSO4, NaoAc, pH 5, VAPOR DIFFUSION, SITTING DROP, temperature 298K
1	VAPOR DIFFUSION, SITTING DROP	5	298	PEG 20K, MnSO4, NaoAc, pH 5, VAPOR DIFFUSION, SITTING DROP, temperature 298K
1	VAPOR DIFFUSION, SITTING DROP	5	298	PEG 20K, MnSO4, NaoAc, pH 5, VAPOR DIFFUSION, SITTING DROP, temperature 298K