2ALL
Crystal structure of L122V/L132V mutant of nitrophorin 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-ID-B |
| Synchrotron site | APS |
| Beamline | 14-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-07-31 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.1808 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 39.440, 126.340, 33.960 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.470 |
| R-factor | 0.13922 |
| Rwork | 0.137 |
| R-free | 0.17730 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1euo |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.854 |
| Data reduction software | XDISPLAYF |
| Data scaling software | d*TREK |
| Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.520 |
| High resolution limit [Å] | 1.470 | 1.470 |
| Number of reflections | 29090 | |
| <I/σ(I)> | 20.8 | 4.8 |
| Completeness [%] | 97.0 | 84 |
| Redundancy | 6.6 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | sodium citrate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
