2ALE
Crystal structure of yeast RNA splicing factor Snu13p
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2004-01-15 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 70.330, 70.330, 53.303 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 14.680 - 1.800 |
R-factor | 0.223 |
Rwork | 0.223 |
R-free | 0.24800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1e7k |
RMSD bond length | 0.005 |
RMSD bond angle | 1.200 |
Data reduction software | CrystalClear ((MSC/RIGAKU)) |
Data scaling software | d*TREK |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.530 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.095 | 0.326 |
Number of reflections | 14642 | |
<I/σ(I)> | 11.8 | 2.8 |
Completeness [%] | 99.4 | 99 |
Redundancy | 5.2 | 5.15 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.2 M Sodium Acetate, 0.1 M Sodium Cacodylate, 30% PEG 8K, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |