2AL7
Structure Of Human ADP-Ribosylation Factor-Like 10C
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-08-03 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.732, 62.393, 63.796 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.590 - 1.850 |
| R-factor | 0.20318 |
| Rwork | 0.201 |
| R-free | 0.25062 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zd9 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.533 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.590 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Number of reflections | 13639 | |
| <I/σ(I)> | 52 | 16.25 |
| Completeness [%] | 79.8 | |
| Redundancy | 4.5 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | PEG 3350 25%, Ammonium Acetate 100mM, 100mM HEPES (7.5), VAPOR DIFFUSION, SITTING DROP, temperature 298K |
