2AKC
Crystal structure of tungstate complex of the PhoN protein from S. typhimurium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-08-03 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 189.800, 45.200, 112.700 |
| Unit cell angles | 90.00, 111.40, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| Rwork | 0.164 |
| R-free | 0.21100 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2a96 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CCP4 |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.060 | 0.110 |
| Number of reflections | 37069 | |
| <I/σ(I)> | 9.8 | 3.2 |
| Completeness [%] | 93.0 | 89.2 |
| Redundancy | 1.8 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | PEG-6000 15%, Glycerol 2%, 1mM sodium tungstate, 25mM sodium acetate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
