2AJH
Crystal structure of the editing domain of E. coli leucyl-tRNA synthetase complexes with methionine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 123 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-03-11 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 111.518, 111.518, 134.931 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.400 |
| R-factor | 0.24331 |
| Rwork | 0.241 |
| R-free | 0.28432 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.474 |
| Phasing software | CNS |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.121 | 0.452 |
| Number of reflections | 19784 | |
| <I/σ(I)> | 4.5 | 1.4 |
| Completeness [%] | 98.9 | 99.7 |
| Redundancy | 6.2 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | (NH4)2SO4, pH 9, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
