2AJF
Structure of SARS coronavirus spike receptor-binding domain complexed with its receptor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Collection date | 2005-03-05 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 82.299, 119.429, 113.237 |
| Unit cell angles | 90.00, 91.97, 90.00 |
Refinement procedure
| Resolution | 47.400 - 2.900 |
| R-factor | 0.221 |
| Rwork | 0.218 |
| R-free | 0.27500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1r42 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.253 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.400 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 41841 | |
| <I/σ(I)> | 20.5 | 1.83 |
| Completeness [%] | 93.1 | 93.1 |
| Redundancy | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 100 mM Tris pH 8.2, 24% PEG6000, 150 mM NaCl, 10% ethylene glycol, VAPOR DIFFUSION, HANGING DROP, pH 7.5, pH 7.50 |
