2AIO

Metallo beta lactamase L1 from Stenotrophomonas maltophilia complexed with hydrolyzed moxalactam

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX14.2
Synchrotron site	SRS
Beamline	PX14.2
Temperature [K]	100
Detector technology	CCD
Collection date	2001-11-30
Detector	ADSC QUANTUM 4
Spacegroup name	P 64 2 2
Unit cell lengths	105.024, 105.024, 98.230
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.990 - 1.700
R-factor	0.173
Rwork	0.171
R-free	0.20000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1sml
RMSD bond length	0.011
RMSD bond angle	1.282
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.740
High resolution limit [Å]	1.700	1.700
Rmerge	0.057	0.246
Number of reflections	33459
<I/σ(I)>	23.69	2.65
Completeness [%]	93.6	78.6
Redundancy	4.35

Crystallization Conditions

crystal ID	method	pH	temperature	details
1