2AIF
Crystal Structure of High Mobility Like Protein, NHP2, putative from Cryptosporidium parvum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-07-23 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97972 |
| Spacegroup name | H 3 |
| Unit cell lengths | 90.703, 90.703, 31.275 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 17.900 - 1.895 |
| R-factor | 0.20335 |
| Rwork | 0.198 |
| R-free | 0.27028 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1e7k |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.279 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 17.900 | 1.930 |
| High resolution limit [Å] | 1.895 | 1.895 |
| Rmerge | 0.068 | 0.397 |
| Number of reflections | 6861 | |
| <I/σ(I)> | 10.7 | 2.01 |
| Completeness [%] | 90.0 | 61.3 |
| Redundancy | 6.7 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 300 | 1.5 M Sodium Citrate and 0.1M Hepes pH 7.5, VAPOR DIFFUSION, temperature 300K |
