2AI3
Purine nucleoside phosphorylase from calf spleen
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 1997-08-28 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.928 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 93.992, 93.992, 93.992 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.330 - 1.700 |
| R-factor | 0.221 |
| Rwork | 0.221 |
| R-free | 0.25300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1a9p |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.400 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 29787 | |
| <I/σ(I)> | 42 | 5 |
| Completeness [%] | 99.3 | 98.3 |
| Redundancy | 8.7 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 296 | PEG2K MME , pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
