2AHY
Na+ complex of the NaK Channel
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-02-11 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 81.528, 85.482, 129.572 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.700 - 2.400 |
| R-factor | 0.236 |
| Rwork | 0.236 |
| R-free | 0.26100 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.140 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.700 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.045 | 0.555 |
| Number of reflections | 17824 | |
| <I/σ(I)> | 35 | 1.8 |
| Completeness [%] | 97.6 | 84.1 |
| Redundancy | 5 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 293 | PEG 400, NaCl, CaCl2, Tris.HCl, t-butanol, n-Decyl-beta-D-maltopyranoside, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293 K, pH 8.00 |
