2AHP
GCN4 leucine zipper, mutation of Lys15 to epsilon-azido-Lys
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-07-01 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 100.162, 30.397, 21.716 |
| Unit cell angles | 90.00, 95.21, 90.00 |
Refinement procedure
| Resolution | 29.080 - 2.000 |
| R-factor | 0.192 |
| Rwork | 0.189 |
| R-free | 0.26300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.204 |
| Data reduction software | CrystalClear ((MSC/RIGAKU)) |
| Data scaling software | d*TREK (8.0SSI) |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.080 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.036 | 0.140 |
| Number of reflections | 4473 | |
| <I/σ(I)> | 10.6 | 3.4 |
| Completeness [%] | 98.7 | 98 |
| Redundancy | 3.49 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295 | 0.065 M Tris, 5.2% w/v PEG 8000, 35% v/v anhydrous glycerol, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
