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2AH8

roGFP1-R7. Cystal structure analysis of a rate-enhanced variant of redox-sensitive green fluorescent protein in the oxidized form.

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeROTATING ANODE
Source detailsRIGAKU RUH3R
Temperature [K]110
Detector technologyIMAGE PLATE
Collection date2003-11-04
DetectorRIGAKU RAXIS IV
Wavelength(s)1.54
Spacegroup nameP 32 2 1
Unit cell lengths126.297, 126.297, 78.412
Unit cell angles90.00, 90.00, 120.00
Refinement procedure
Resolution24.000 - 2.240
R-factor0.202
Rwork0.196
R-free0.28000
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)1ema
RMSD bond length0.014
RMSD bond angle2.600
Data reduction softwareDENZO
Data scaling softwareSCALEPACK
Phasing softwareEPMR (2.5)
Refinement softwareTNT
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]40.0002.280
High resolution limit [Å]2.2002.200
Rmerge0.0960.634
Number of reflections36457
Completeness [%]99.999.9
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP7.6295sodium citrate, imidazole, pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 295K

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