2AGJ
Crystal Structure of a glycosylated Fab from an IgM cryoglobulin with properties of a natural proteolytic antibody
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Collection date | 2002-08-14 |
Detector | MARRESEARCH |
Wavelength(s) | 1.5418 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 82.433, 103.653, 138.715 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.200 - 2.600 |
R-factor | 0.225 |
Rwork | 0.219 |
R-free | 0.27900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1IGM 1DN0 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.800 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 99.000 | 2.690 |
High resolution limit [Å] | 2.600 | 2.600 |
Number of reflections | 18260 | |
<I/σ(I)> | 32.5 | 2 |
Completeness [%] | 98.0 | 95.7 |
Redundancy | 8.5 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | PEG 15000-20000, sodium chloride, MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |