2AG8
NADP complex of Pyrroline-5-carboxylate reductase from Neisseria meningitidis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-08-23 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.97917 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 89.701, 49.826, 65.445 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.100 |
| R-factor | 0.18099 |
| Rwork | 0.177 |
| R-free | 0.25244 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yqg |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.937 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MERLOT |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.086 | 0.391 |
| Number of reflections | 14057 | |
| <I/σ(I)> | 14.1 | 1.72 |
| Completeness [%] | 78.4 | 35.2 |
| Redundancy | 2.7 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | Ammonium sulfate, HEPES, PEG 3350, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
