2AFY
Formylglycine generating enzyme C341S mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2005-06-08 |
Detector | MARRESEARCH |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 61.873, 109.524, 43.374 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.460 - 1.490 |
R-factor | 0.14067 |
Rwork | 0.139 |
R-free | 0.17130 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1y1i |
RMSD bond length | 0.011 |
RMSD bond angle | 1.328 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | REFMAC (5.2.0009) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.530 |
High resolution limit [Å] | 1.480 | 1.480 |
Number of reflections | 45114 | |
<I/σ(I)> | 23.3 | 2.4 |
Completeness [%] | 89.9 | 25.9 |
Redundancy | 3.7 | 1.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | PEG 4000, Calcium chloride, TRIS, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |