2AFN

STRUCTURE OF ALCALIGENES FAECALIS NITRITE REDUCTASE AND A COPPER SITE MUTANT, M150E, THAT CONTAINS ZINC

Replaces:  1AFN

Experimental procedure

Detector technology	IMAGE PLATE
Detector	RIGAKU
Spacegroup name	P 21 21 21
Unit cell lengths	63.035, 103.610, 146.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.000
R-factor	0.158
Rwork	0.158
R-free	0.22300
RMSD bond length	0.009
RMSD bond angle	27.300 *
Data reduction software	RIGAKU
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	85.000
High resolution limit [Å]	2.000	2.000 *
Rmerge	0.051	0.154 *
Number of reflections	55751	13333 *
Completeness [%]	85.0 *	69 *
Redundancy	3.2	2.3 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	MES	10 (mM)
3	1	drop	reservoir
4	1	reservoir	PEG4000	10-25 (%)
5	1	reservoir	sodium acetate	0.1 (M)