2AF5
2.5A X-ray Structure of Engineered OspA protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-ID-B |
Synchrotron site | APS |
Beamline | 14-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-10-15 |
Detector | MARRESEARCH |
Wavelength(s) | 0.97946 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 48.547, 55.330, 70.361 |
Unit cell angles | 90.00, 104.08, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.500 |
R-factor | 0.24572 |
Rwork | 0.242 |
R-free | 0.27556 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1osp |
RMSD bond length | 0.013 |
RMSD bond angle | 1.481 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.1.9999) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.000 |
High resolution limit [Å] | 2.400 |
Rmerge | 0.075 |
Number of reflections | 14271 |
<I/σ(I)> | 9.2 |
Completeness [%] | 99.9 |
Redundancy | 5.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |