2AEP

An epidemiologically significant epitope of a 1998 influenza virus neuraminidase forms a highly hydrated interface in the NA-antibody complex.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2003-06-11
Detector	RIGAKU RAXIS IV
Spacegroup name	P 4 21 2
Unit cell lengths	155.057, 155.057, 102.645
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.100
R-factor	0.19
Rwork	0.188
R-free	0.22400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1nn2 1nca
RMSD bond length	0.025
RMSD bond angle	1.986
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.170
High resolution limit [Å]	2.100	2.100
Number of reflections	73146
<I/σ(I)>	17.64	2.06
Completeness [%]	100.0	100
Redundancy	6.25	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.5		2M AMMONIUM SULFATE, 100 mM TRIS, pH 7.5