2AEN
Crystal structure of the rotavirus strain DS-1 VP8* core
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-02-13 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9160 |
Spacegroup name | P 1 |
Unit cell lengths | 42.421, 84.123, 90.769 |
Unit cell angles | 90.03, 90.02, 75.54 |
Refinement procedure
Resolution | 14.880 - 1.604 |
R-factor | 0.15961 |
Rwork | 0.158 |
R-free | 0.19173 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1kqr |
RMSD bond length | 0.011 |
RMSD bond angle | 1.310 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | REFMAC (5.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 14.880 | 1.660 |
High resolution limit [Å] | 1.600 | 1.600 |
Number of reflections | 153466 | |
<I/σ(I)> | 17.2 | 3.4 |
Completeness [%] | 96.8 | 94.7 |
Redundancy | 1.95 | 1.72 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | PEG 4000, sodium chloride, sodium citrate, ethanol, tris, EDTA, sodium azide, benzamidine, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |