2AEJ
Crystal Structures of the MthK RCK Domain in no Ca2+ bound form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-12-10 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9911 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.900, 38.167, 96.700 |
| Unit cell angles | 90.00, 95.95, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.100 |
| R-factor | 0.21 |
| Rwork | 0.210 |
| R-free | 0.24470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lnq |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.052 | 0.384 |
| Number of reflections | 24915 | |
| <I/σ(I)> | 24 | 3.35 |
| Completeness [%] | 99.7 | 98.8 |
| Redundancy | 3.6 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 293 | PEG 4000, Ammonium formate, sodium acetate , pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
