2AEH

Focal adhesion kinase 1

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F2
Synchrotron site	CHESS
Beamline	F2
Temperature [K]	100
Detector technology	CCD
Collection date	2002-05-03
Detector	CUSTOM-MADE
Wavelength(s)	0.9796,0.9793,0.95004
Spacegroup name	P 1 21 1
Unit cell lengths	44.213, 146.231, 68.919
Unit cell angles	90.00, 96.37, 90.00

Refinement procedure

Resolution	20.000 - 2.530
R-factor	0.21
Rwork	0.206
R-free	0.24900
Structure solution method	MAD
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.600	2.570
High resolution limit [Å]	2.500	2.530
Rmerge	0.073
Number of reflections	29870
<I/σ(I)>	22.5	2.7
Completeness [%]	100.0	100
Redundancy	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	298	100 mM Tris pH 8.0, 16-22% PEG 4000, 20% glycerol, 200 mM NaCl, 100 mM CsCl, 10 mM DTT, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	8	298	100 mM Tris pH 8.0, 16-22% PEG 4000, 20% glycerol, 200 mM NaCl, 100 mM CsCl, 10 mM DTT, VAPOR DIFFUSION, HANGING DROP, temperature 298K