2AEG
X-Ray Crystal Structure of Protein Atu5096 from Agrobacterium tumefaciens. Northeast Structural Genomics Consortium Target AtR63.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-06-18 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97913 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 195.926, 73.994, 65.566 |
| Unit cell angles | 90.00, 106.21, 90.00 |
Refinement procedure
| Resolution | 29.810 - 2.300 |
| R-factor | 0.217 |
| Rwork | 0.217 |
| R-free | 0.25400 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.071 | 0.262 |
| Number of reflections | 77934 | |
| <I/σ(I)> | 15.61 | 4.85 |
| Completeness [%] | 99.2 | 99.5 |
| Redundancy | 3.8 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 100 mM Bis-Tris (pH 5.5), 20% PEG 3350, 220 mM (NH)4 Acetate, and 5 mM DTT., VAPOR DIFFUSION, HANGING DROP, temperature 293K |
