2AEF
Crystal Structures of the MthK RCK Domain in Ca2+ bound form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-03-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.979, 37.908, 96.652 |
| Unit cell angles | 90.00, 95.49, 90.00 |
Refinement procedure
| Resolution | 29.200 - 1.700 |
| R-factor | 0.23 |
| Rwork | 0.228 |
| R-free | 0.26800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lnq |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.200 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.030 | 0.030 |
| Number of reflections | 46246 | |
| <I/σ(I)> | 40.8 | |
| Completeness [%] | 99.2 | 98.1 |
| Redundancy | 4.1 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 293 | PEG 4000, calcium chloride, Ammonium formate, sodium acetate, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
