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2AEC

Crystal Structure of Human M340H-Beta1,4-Galactosyltransferase-I (M340H-B4GAL-T1) in Complex with GlcNAc-beta1,2-Man-alpha1,6-Man-beta-OR

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsNSLS BEAMLINE X9B
Synchrotron siteNSLS
BeamlineX9B
Temperature [K]100
Detector technologyCCD
Collection date2004-02-20
DetectorADSC QUANTUM 4
Wavelength(s)1.0402
Spacegroup nameC 2 2 21
Unit cell lengths107.228, 194.990, 143.744
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution40.000 - 2.000
R-factor0.208
Rwork0.208
R-free0.23900
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)1o0r
RMSD bond length0.010
RMSD bond angle1.500
Data reduction softwareHKL-2000
Data scaling softwareSCALEPACK
Phasing softwareAMoRE
Refinement softwareCNS (1.1)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]40.0002.070
High resolution limit [Å]2.0002.000
Number of reflections100723
<I/σ(I)>26.63.8
Completeness [%]99.699.3
Redundancy5.65.5
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP6.5298mes, ammonium sulfate, dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K

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