2AE6
Crystal Structure of Acetyltransferase of GNAT family from Enterococcus faecalis V583
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-02-07 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97910 |
Spacegroup name | P 32 |
Unit cell lengths | 64.618, 64.618, 176.396 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 58.820 - 2.190 |
R-factor | 0.2152 |
Rwork | 0.210 |
R-free | 0.25929 |
Structure solution method | SAD |
RMSD bond length | 0.012 |
RMSD bond angle | 1.323 |
Data scaling software | SCALEPACK |
Phasing software | SHELX |
Refinement software | REFMAC (5.2.0000) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 58.820 | 2.280 |
High resolution limit [Å] | 2.190 | 2.190 |
Rmerge | 0.081 | 0.613 |
Number of reflections | 40808 | |
<I/σ(I)> | 12.7 | 1.8 |
Completeness [%] | 96.9 | 90.3 |
Redundancy | 9.8 | 5.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 295 | ETHANOL, HEPES, Magnesium Chloride, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K |