2ADO
Crystal Structure Of The Brct Repeat Region From The Mediator of DNA damage checkpoint protein 1, MDC1
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-07-29 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.1159,0.9795471,1.019876 |
Spacegroup name | P 1 |
Unit cell lengths | 42.051, 44.440, 61.942 |
Unit cell angles | 72.95, 87.54, 61.80 |
Refinement procedure
Resolution | 30.000 - 1.450 |
Rwork | 0.195 |
R-free | 0.21200 |
Structure solution method | MAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.700 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE (2.08) |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.500 |
High resolution limit [Å] | 1.450 | 1.450 |
Rmerge | 0.048 | 0.380 |
Number of reflections | 60634 | |
Completeness [%] | 90.8 | 75.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | PEG 8000, HEPES, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | PEG 8000, HEPES, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |