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2ADO

Crystal Structure Of The Brct Repeat Region From The Mediator of DNA damage checkpoint protein 1, MDC1

Experimental procedure
Experimental methodMAD
Source typeSYNCHROTRON
Source detailsALS BEAMLINE 8.3.1
Synchrotron siteALS
Beamline8.3.1
Temperature [K]100
Detector technologyCCD
Collection date2004-07-29
DetectorADSC QUANTUM 210
Wavelength(s)1.1159,0.9795471,1.019876
Spacegroup nameP 1
Unit cell lengths42.051, 44.440, 61.942
Unit cell angles72.95, 87.54, 61.80
Refinement procedure
Resolution30.000 - 1.450
Rwork0.195
R-free0.21200
Structure solution methodMAD
RMSD bond length0.010
RMSD bond angle1.700
Data reduction softwareDENZO
Data scaling softwareSCALEPACK
Phasing softwareSOLVE (2.08)
Refinement softwareCNS
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]30.0001.500
High resolution limit [Å]1.4501.450
Rmerge0.0480.380
Number of reflections60634
Completeness [%]90.875.9
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP8298PEG 8000, HEPES, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
1VAPOR DIFFUSION, HANGING DROP8298PEG 8000, HEPES, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K

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