2ADI
Crystal structure of monoclonal anti-CD4 antibody Q425 in complex with Barium
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 298 |
Detector technology | AREA DETECTOR |
Collection date | 1994-01-26 |
Detector | XUONG-HAMLIN MULTIWIRE |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 97.200, 97.200, 110.300 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 9.990 - 2.800 |
R-factor | 0.21 |
Rwork | 0.210 |
R-free | 0.26900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2adg |
RMSD bond length | 0.007 |
RMSD bond angle | 1.400 |
Data reduction software | XUONG-HAMLIN ((DETECTOR SYSTEM)) |
Data scaling software | CCP4 ((AGROVATA) |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 10.000 | 2.920 |
High resolution limit [Å] | 2.800 | 2.800 |
Number of reflections | 12858 | |
<I/σ(I)> | 5.5 | |
Completeness [%] | 97.4 | 78 |
Redundancy | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | PEG4000, Sodium Acetate, Barium, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |