2ADI

Crystal structure of monoclonal anti-CD4 antibody Q425 in complex with Barium

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	298
Detector technology	AREA DETECTOR
Collection date	1994-01-26
Detector	XUONG-HAMLIN MULTIWIRE
Spacegroup name	P 43 21 2
Unit cell lengths	97.200, 97.200, 110.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	9.990 - 2.800
R-factor	0.21
Rwork	0.210
R-free	0.26900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2adg
RMSD bond length	0.007
RMSD bond angle	1.400
Data reduction software	XUONG-HAMLIN ((DETECTOR SYSTEM))
Data scaling software	CCP4 ((AGROVATA)
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	10.000	2.920
High resolution limit [Å]	2.800	2.800
Number of reflections	12858
<I/σ(I)>	5.5
Completeness [%]	97.4	78
Redundancy	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	298	PEG4000, Sodium Acetate, Barium, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K