2AD8
crystal structure of methanol dehydrogenase from M. W3A1 (form C) in the presence of ethanol
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-6A |
Synchrotron site | Photon Factory |
Beamline | BL-6A |
Temperature [K] | 293 |
Detector technology | CCD |
Collection date | 2002-12-02 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 204.205, 62.630, 124.511 |
Unit cell angles | 90.00, 125.14, 90.00 |
Refinement procedure
Resolution | 42.990 - 1.600 |
R-factor | 0.174 |
Rwork | 0.174 |
R-free | 0.19500 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.005 |
RMSD bond angle | 1.400 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.660 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.046 | 0.219 |
Number of reflections | 157861 | |
Completeness [%] | 93.3 | 68.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | macroseeding | 8.25 | 293 | Tris-HCl, PEG 8000, ethanol, pH 8.25, macroseeding, temperature 293K |