2AD1
Human Sulfotransferase SULT1C2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-05-29 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 58.824, 58.824, 189.287 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.000 |
| R-factor | 0.21993 |
| Rwork | 0.218 |
| R-free | 0.26166 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ZHE |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.703 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.059 | 0.767 |
| Number of reflections | 25032 | |
| <I/σ(I)> | 14.6 | 2.89 |
| Completeness [%] | 93.7 | 72.5 |
| Redundancy | 10.7 | 9.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.6 | 300 | 20%PEG 3350, 0.2M Li citracte pH4.6, VAPOR DIFFUSION, temperature 300K |
