2ACV
Crystal Structure of Medicago truncatula UGT71G1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH3R |
Temperature [K] | 93 |
Detector technology | IMAGE PLATE |
Collection date | 2005-01-03 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 53.713, 90.593, 101.788 |
Unit cell angles | 90.00, 102.57, 90.00 |
Refinement procedure
Resolution | 66.940 - 2.000 |
R-factor | 0.206 |
Rwork | 0.187 |
R-free | 0.22900 |
Structure solution method | MAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.270 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.041 | 0.277 |
Number of reflections | 62825 | |
<I/σ(I)> | 13.7 | 2.7 |
Completeness [%] | 98.2 | 97.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | PEG 3350, ammonium acetate, sodium citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | PEG 3350, ammonium acetate, sodium citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |