2ACJ
Crystal structure of the B/Z junction containing DNA bound to Z-DNA binding proteins
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-11-08 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97939, 0.97952, 0.97171 |
| Spacegroup name | P 61 |
| Unit cell lengths | 110.765, 110.765, 61.762 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.600 |
| R-factor | 0.24254 |
| Rwork | 0.238 |
| R-free | 0.28486 |
| Structure solution method | MAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.498 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 13389 | |
| Completeness [%] | 99.3 | 81.4 |
| Redundancy | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 295 | 22-23% MPD, 55-60mM sodium acetate, 15-16mM calsium chloride, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MPD | ||
| 2 | 1 | 1 | sodium acetate | ||
| 3 | 1 | 1 | calsium chloride | ||
| 4 | 1 | 2 | MPD | ||
| 5 | 1 | 2 | sodium acetate | ||
| 6 | 1 | 2 | calsium chloride |
