2AC7
Crystal structure of Adenosine Phosphorylase from Bacillus cereus with adenosine bound in the active site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2004-03-06 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9756 |
| Spacegroup name | P 63 |
| Unit cell lengths | 122.000, 122.000, 68.000 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 15.000 - 1.700 |
| R-factor | 0.17835 |
| Rwork | 0.177 |
| R-free | 0.20223 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.292 |
| Data reduction software | DENZO |
| Data scaling software | XDS |
| Phasing software | CNS |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 15.000 |
| High resolution limit [Å] | 1.600 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | Ammonium sulfate, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
