2AC4
Crystal structure of the His183Cys mutant variant of Bacillus subtilis Ferrochelatase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-10-14 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.094 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.700, 49.800, 119.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.100 |
| R-factor | 0.214 |
| Rwork | 0.214 |
| R-free | 0.27600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1doz |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data scaling software | XDS |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.230 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.090 | 0.227 |
| Number of reflections | 16999 | |
| Completeness [%] | 97.1 | 88.9 |
| Redundancy | 4.5 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.4 | 298 | PEG 2000, magnesium chloride, tris, pH 7.4, VAPOR DIFFUSION, temperature 298K |
