2AB0
Crystal Structure of E. coli protein YajL (ThiJ)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2004-04-23 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.86 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 42.722, 78.475, 99.946 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 100.000 - 1.100 |
| R-factor | 0.1366 |
| Rwork | 0.136 |
| R-free | 0.17060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1p5f |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.027 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.140 |
| High resolution limit [Å] | 1.100 | 1.100 |
| Rmerge | 0.063 | 0.741 |
| Number of reflections | 132051 | |
| <I/σ(I)> | 33.27 | 2.24 |
| Completeness [%] | 96.4 | 92.9 |
| Redundancy | 7 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | PEG4000, Tris-HCl, magnesium chloride, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
