2AAR
Structure of trigger factor binding domain in biologically homologous complex with eubacterial ribosome.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Detector technology | CCD |
| Collection date | 2005-04-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97934 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 169.386, 407.063, 692.746 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 3.500 |
| R-factor | 0.267 |
| Rwork | 0.251 |
| R-free | 0.32000 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1nkw |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.300 |
| Data reduction software | TRUNCATE |
| Data scaling software | CCP4 ((TRUNCATE)) |
| Phasing software | CCP4 |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 3.500 |
| Number of reflections | 294915 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 293 | pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 20K |
