2AAO
Regulatory apparatus of Calcium Dependent protein kinase from Arabidopsis thaliana
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-05-20 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9766, 0.9795, 0.95 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 35.968, 88.154, 117.552 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 14.700 - 2.000 |
R-factor | 0.278 |
Rwork | 0.213 |
R-free | 0.25400 |
Structure solution method | MAD |
RMSD bond length | 0.017 |
RMSD bond angle | 1.308 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | REFMAC (5.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.030 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.095 | 0.167 |
Number of reflections | 25329 | |
<I/σ(I)> | 19.5 | 2.6 |
Completeness [%] | 97.3 | 86.7 |
Redundancy | 9.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 293 | PEG400, calcium chloride, sodium acetate , pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |