2AAN
Auracyanin A: A "blue" copper protein from the green thermophilic photosynthetic bacterium,chloroflexus aurantiacus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-5 |
| Synchrotron site | MAX II |
| Beamline | I911-5 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-03-07 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.96868 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 70.578, 70.578, 45.665 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 18.000 - 1.850 |
| R-factor | 0.16436 |
| Rwork | 0.162 |
| R-free | 0.20703 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | auracyanin B |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.586 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.898 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.086 | 0.320 |
| Number of reflections | 9667 | |
| Completeness [%] | 95.6 | 99.3 |
| Redundancy | 10.8 | 10 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 277 | Lithium sulfate, sodium citrate, pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
